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Morphofunctional and ultrastructural analysis of the nuclear envelope and chromatin

 

Staff: Giovanna Lattanzi, Stefano Squarzoni, Cristina Capanni, Elisabetta Mattioli, Vittoria Cenni, Rosalba Del Coco.

 

In recent years the use of optical microscopy associated to molecular biology has led to the identification of several functional domains that constitute the nucleus of mammalian cells. The analysis of the distribution of proteins in the nucleus is particularly interesting given that the space-time regulation of the biochemical processes in eukaryotes is obtained thanks to a high degree of compartmentalization. Over 10 hereditary disorders have recently been associated with mutations in nuclear envelope proteins or related proteins. These diseases have been defined as Laminopathies. With this service we want to offer our expertise in support of research groups who work occasionally or who want to start studying nuclear proteins involved in genetic diseases.

 

 

 

 


Scanning transmission electron microscopy

Staff: Stefano Squarzoni, Nicoletta Zini, Patrizia Sabatelli.

 

The use of electron microscopy associated to molecular biology has led to the identification of sub-nuclear domains involved in the organization and chromatin remodeling, in DNA replication and RNA production. The ultrastructural analysis has allowed the identification of alterations in the nuclear pathologies with defect of nuclear lamina and in some muscular dystrophies of different origin.

The analysis of very high resolution transmission electron microscopy of muscle tissue allows the identification of ultrastructural abnormalities in hereditary and acquired muscular disorders, including the muscular dystrophies.

The use of electron microscopy associated with cell biology has led to the identification of some of the mechanisms of stem cell growth on biomaterial implant.

The use of scanning electron microscopy associated with microanalysis is used for the analysis of wear for orthopedic implants.

 

 

 


Primary cell cultures

Staff: Elisabetta Mattioli, Giovanna Lattanzi, Cristina Capanni, Vittoria Cenni, Rosalba Del Coco.

 

IGM-CNR has a laboratory for the preparation, expansion and stortage of cell cultures from human biopsy tissues. Cultured myoblasts, muscle and skin fibroblasts, pre-adipocytes can be produced. The cultures are tested for mycoplasma contamination. The storage of samples occurs in liquid nitrogen.

 


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