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DEVELOPMENT OF INNOVATIVE OPTICAL TECHNIQUES AND METHODS IN BIOLOGY AND BIOMEDICAL APPLICATIONS

 

Croce Anna Cleta

 

Native fluorescence, or autofluorescence from cells and tissues, consists in the emission of light in the UV-visible, near-IR spectral range when naturally present biomolecules are excited with light at suitable wavelength. The common presence of several intrinsic biomolecules acting as endogenous fluorophores in the organisms of the whole life kingdom makes autofluorescence a widespread phenomenon.  

 

 




 

 

Our interest  is given to the animal kingdom, to use endogenous fluorophores  for the the real time, in situ detection and diagnosis of normal, altered and diseased conditions of cells and tissues.

 

 




 

In fact, the strict relationship of nature, amount and autofluorescence emission properties of many endogenous fluorophores with metabolic engagement and structural properties of cells and tissues is an extremely powerful resource for their direct characterization and monitoring of metabolism alterations in the absence of exogenous markers and sample removal.  

 




 

The coenzymes NAD(P)H and flavins are since a long time used as autofluorescence biomarkers for cell and tissue redox state changes, engagement in aerobic/anaerobic metabolism, and thus of mitochondrial functionality and, for example, responses to ischemic conditions.

 




 

At present, our activity is mainly devoted to a comprehensive autofluorescence investigation of liver to detect energy metabolism alterations, excess of lipids and oxidative stress, and functionality impairment, for a sensitive, real time, in situ early diagnosis of disorders, pre-selection of marginal livers as donor organ suitable for transplantation and monitoring of functional impairment during organ preservation. 

 




 

In fact, since oxidative stress consequent to the excess of lipids is further increasing the risk of tissue injury during donor organ preservation, our aim is to validate and propose autofluorescence as a real time, in situ diagnostic supportive tool in experimental hepatology engaged to improve preservation procedures and ameliorate the outcome of marginal donor livers.

 

Bibliografia

Croce A.C., et al. Lasers Surg. Med., 371-378, 42, 2010.

Croce A.C., et al. Photochem Photobiol Sci., 1189-1195, 10, 2011.

Santin G. et al. Lasers Surg. Med., 597-607, 45, 2013.

Croce A.C., et al. J. Biophotonics. 7, 810-817, 2014.

Croce A.C., et al. Lasers Surg. Med., 412-421, 46, 2014

Croce A.C., et al.  BioMed. Res. Int., Vol. 2014, Article ID 803491 doi:10.1155/2014/803491, 2014;

Croce A.C., et al. Eur. J. Histochem., 2015 in press

 


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