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HIV drug resistance


 

Current anti-HIV drugs targeted to the reverse transcriptase are severly limited by the development of drug resistance. We are currently undertaking a sytematic evaluation of the impact of drug resistance mutations in the viral RT on the viral fitness and drug sensitivity. We clone multi-resistant HIV-1 RTs from clinical isolates coming from AIDS patients failing therapy, express and purify the recombinant enzymes and evaluate their enzymological parameters. With this approach we already identified uncommon inter-class drug resistance patterns for mutations such as Y181I/C, Q145M, G190S, T215Y (1,2).

 


References

  1. Blanca et al., J. Biol. Chem. 2003;278:15469-72
  2. Paolucci et al., AIDS 2003;17:924-7


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