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Enzymology of DNA replication

Giovanni Maga

 

We are interested in understanding the complex relationships between the DNA synthetic machinery (replisome) and other aspects of cell metabolism such as DNA repair, DNA damage tolerance and cell cycle regulation. We have set up a protocol for the isolation of multiprotein complexes from the nuclei of HeLa cells which turned out to be DNA replication/repair proficient and to contain cell cycle regulatory proteins (1).
We are also studying the roles of the auxiliary proteins RP-A and PCNA on the mechanisms of DNA damage tolerance. We have shown that PCNA and RP-A largely determine the ability of various cellular DNA polymerases to cope with DNA lesions such as abasic sites and 8-oxo-dG (2).




References

  1. Frouin et al., EMBO J. 2002;21:2485-95
  2. Maga et al., Nature. 2007;447:606-8


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