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Cell-protection mechanism through autophagy in HGFs/S. mitis co-culture treated with Chitlac-nAg.

Autori

Gallorini M, di Giacomo V, Di Valerio V, Rapino M, Bosco D, Travan A, Di Giulio M, Di Pietro R, Paoletti S, Cataldi A, Sancilio S.

Riferimenti

JOURNAL OF MATERIALS SCIENCE. MATERIALS IN MEDICINE. 27(12) 186-, 2016

Autori CNR

BOSCO, RAPINO

Moduli

Abstract

Silver-based products have been proven to be effective in retarding and preventing bacterial growth since ancient times. In the field of restorative dentistry, the use of silver ions/nanoparticles has been explored to counteract bacterial infections, as silver can destroy bacterial cell walls by reacting with membrane proteins. However, it is also cytotoxic towards eukaryotic cells, which are capable of internalizing nanoparticles. In this work, we investigated the biological effects of Chitlac-nAg, a colloidal system based on a modified chitosan (Chitlac), administered for 24-48_h to a co-culture of primary human gingival fibroblasts and Streptococcus mitis in the presence of saliva, developed to mimic the microenvironment of the oral cavity. We sought to determine its efficiency to combat oral hygiene-related diseases without affecting eukaryotic cells. Cytotoxicity, reactive oxygen species production, apoptosis induction, nanoparticles uptake, and lysosome and autophagosome metabolism were evaluated. In vitro results show that Chitlac-nAg does not exert cytotoxic effects on human gingival fibroblasts, which seem to survive through a homoeostasis mechanism involving autophagy. That suggests that the novel biomaterial Chitlac-nAg could be a promising tool in the field of dentistry.

Link all articolo

http://link.springer.com/article/10.1007%2Fs10856-016-5803-5

Parole Chiave

Note

10.1007/s10856-016-5803-5

Indietro


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