Montecucco A; Zanetta F; Biamonti G Molecular mechanisms of etoposide. Journal Article In: EXCLI Journal, vol. 14, pp. 95-108, 2015. @article{%a1:%Y_382,
title = {Molecular mechanisms of etoposide.},
author = {Montecucco A and Zanetta F and Biamonti G},
url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4652635/},
doi = {10.17179/excli2015-561},
year = {2015},
date = {2015-01-19},
journal = {EXCLI Journal},
volume = {14},
pages = {95-108},
abstract = {Etoposide derives from podophyllotoxin, a toxin found in the American Mayapple. It was first synthesized in
1966 and approved for cancer therapy in 1983 by the U.S. Food and Drug Administration (Hande, 1998). Starting from 1980s several studies demonstrated that etoposide targets DNA topoisomerase II activities thus leading to the production of DNA breaks and eliciting a response that affects several aspects of cell metabolisms. In this review we will focus on molecular mechanisms that account for the biological effect of etoposide.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Etoposide derives from podophyllotoxin, a toxin found in the American Mayapple. It was first synthesized in
1966 and approved for cancer therapy in 1983 by the U.S. Food and Drug Administration (Hande, 1998). Starting from 1980s several studies demonstrated that etoposide targets DNA topoisomerase II activities thus leading to the production of DNA breaks and eliciting a response that affects several aspects of cell metabolisms. In this review we will focus on molecular mechanisms that account for the biological effect of etoposide. |
Lisa A; Astolfi P; Zei G; Tentoni S Consanguinity and late fertility: spatial analysis reveals positive association patterns. Journal Article In: Annals of Human Genetics, vol. 79, no 1, pp. 37-45, 2015. @article{%a1:%Y_350,
title = {Consanguinity and late fertility: spatial analysis reveals positive association patterns.},
author = {Lisa A and Astolfi P and Zei G and Tentoni S},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/ahg.12092},
doi = {10.1111/ahg.12092},
year = {2015},
date = {2015-01-17},
journal = {Annals of Human Genetics},
volume = {79},
number = {1},
pages = {37-45},
abstract = {The role of consanguinity on human complex traits is an important and controversial issue. In this work we focused on the Sardinian population and examined the effect of consanguineous unions on late female fertility. During the last century the island has been characterized by a high incidence of marriages between relatives, favoured by socio economic conditions and geographical isolation, and by high fertility despite a widespread tendency to delay reproduction. Through spatial analysis techniques, we explored the geographical heterogeneity of consanguinity and late fertility, and identified in Central-Eastern Sardinia a common area with an excess of both traits, where the traits are positively associated. We found that their association did not significantly affect women's fertility in the area, despite the expected negative role of both traits. Intriguingly, this critical zone corresponds well to areas reported by previous studies as being peculiar for a high frequency of centenarians and for lower risk in pregnancy outcome. The proposed approach can be generally exploited to identify target populations on which socioeconomic, biodemographic and genetic data can be collected at the individual level, and deeper analyses carried out to disentangle the determinants of complex biological traits and to investigate their association. 2014 John Wiley & Sons Ltd/University College London.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The role of consanguinity on human complex traits is an important and controversial issue. In this work we focused on the Sardinian population and examined the effect of consanguineous unions on late female fertility. During the last century the island has been characterized by a high incidence of marriages between relatives, favoured by socio economic conditions and geographical isolation, and by high fertility despite a widespread tendency to delay reproduction. Through spatial analysis techniques, we explored the geographical heterogeneity of consanguinity and late fertility, and identified in Central-Eastern Sardinia a common area with an excess of both traits, where the traits are positively associated. We found that their association did not significantly affect women's fertility in the area, despite the expected negative role of both traits. Intriguingly, this critical zone corresponds well to areas reported by previous studies as being peculiar for a high frequency of centenarians and for lower risk in pregnancy outcome. The proposed approach can be generally exploited to identify target populations on which socioeconomic, biodemographic and genetic data can be collected at the individual level, and deeper analyses carried out to disentangle the determinants of complex biological traits and to investigate their association. 2014 John Wiley & Sons Ltd/University College London. |
Cansolino L; Clerici AM; Zonta C; Dionigi P; Mazzini G; Di Liberto R; Altieri S; Ballarini F; Bortolussi S; Carante MP; Ferrari M; Gonzalez SJ; Postuma I; Protti N; Santa Cruz GA; Ferrari C Comparative study of the radiobiological effects induced on adherent vs suspended cells by BNCT, neutrons and gamma rays treatments. Journal Article In: Applied Radiation and Isotopes, vol. 106, pp. 226-232, 2015. @article{%a1:%Y_349,
title = {Comparative study of the radiobiological effects induced on adherent vs suspended cells by BNCT, neutrons and gamma rays treatments.},
author = {Cansolino L and Clerici AM and Zonta C and Dionigi P and Mazzini G and {Di Liberto R} and Altieri S and Ballarini F and Bortolussi S and Carante MP and Ferrari M and Gonzalez SJ and Postuma I and Protti N and Santa Cruz GA and Ferrari C},
url = {https://www.sciencedirect.com/science/article/pii/S0969804315301366?via%3Dihub},
doi = {10.1016/j.apradiso.2015.07.054},
year = {2015},
date = {2015-01-08},
journal = {Applied Radiation and Isotopes},
volume = {106},
pages = {226-232},
abstract = {The present work is part of a preclinical in vitro study to assess the efficacy of BNCT applied to liver or lung coloncarcinoma metastases and to limb osteosarcoma. Adherent growing cell lines can be irradiated as adherent to the culture flasks or as cell suspensions, differences in radio-sensitivity of the two modalities of radiation exposure have been investigated. Dose related cell survival and cell cycle perturbation results evidenced that the radiosensitivity of adherent cells is higher than that of the suspended ones.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The present work is part of a preclinical in vitro study to assess the efficacy of BNCT applied to liver or lung coloncarcinoma metastases and to limb osteosarcoma. Adherent growing cell lines can be irradiated as adherent to the culture flasks or as cell suspensions, differences in radio-sensitivity of the two modalities of radiation exposure have been investigated. Dose related cell survival and cell cycle perturbation results evidenced that the radiosensitivity of adherent cells is higher than that of the suspended ones. |
Piva R; Lambertini E; Manferdini C; Capanni C; Penolazzi L; Gabusi E; Paolella F; Lolli A; Angelozzi M; Lattanzi G; Lisignoli G Slug transcription factor and nuclear Lamin B1 are upregulated in osteoarthritic chondrocytes. Journal Article In: Osteoarthritis and Cartilage, vol. 23, no 7, pp. 1226-1230, 2015. @article{%a1:%Y_406,
title = {Slug transcription factor and nuclear Lamin B1 are upregulated in osteoarthritic chondrocytes.},
author = {Piva R and Lambertini E and Manferdini C and Capanni C and Penolazzi L and Gabusi E and Paolella F and Lolli A and Angelozzi M and Lattanzi G and Lisignoli G},
url = {https://www.sciencedirect.com/science/article/pii/S1063458415008560?via%3Dihub},
doi = {10.1016/j.joca.2015.03.015},
year = {2015},
date = {2015-01-01},
urldate = {2015-01-01},
journal = {Osteoarthritis and Cartilage},
volume = {23},
number = {7},
pages = {1226-1230},
abstract = {OBJECTIVE: To contribute to clarify molecular mechanisms supporting senescence and de-differentiation of chondrocytes in chondrocyte pathologies such as osteoarthritis (OA). Specifically, we investigated the relationship between the nuclear lamina protein Lamin B1 and the negative regulator of chondrogenesis Slug transcription factor in osteoarthritic chondrocytes. METHODS: Lamin B1 and Slug proteins were analyzed in cartilage explants from normal subjects and OA patients by immunohistochemical technique. Their expression was confirmed on isolated chondrocytes both at passage 0 and passage 2 (de-differentiated chondrocytes) by immunofluorescence and western blot. Subsequently, we explored the "in vivo" binding of Slug on LMNB1 promoter by chromatin immunoprecipitation assay (ChIP). RESULTS: In this study we demonstrated that nuclear lamina protein Lamin B1 and anti-chondrogenic Slug transcription factor are upregulated in cartilage and OA chondrocytes. Furthermore, we found that Slug is "in vivo" recruited by LMNB1 gene promoter mostly when chondrocytes undergo de-differentiation or OA degeneration. CONCLUSIONS: We described for the first time a potential regulatory role of Slug on the LMNB1 gene expression in OA chondrocytes. These findings may have important implications for the study of premature senescence, and degeneration of cartilage, and may contribute to develop effective therapeutic strategies against signals supporting cartilage damage in different subsets of patients. Copyright 2015 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
OBJECTIVE: To contribute to clarify molecular mechanisms supporting senescence and de-differentiation of chondrocytes in chondrocyte pathologies such as osteoarthritis (OA). Specifically, we investigated the relationship between the nuclear lamina protein Lamin B1 and the negative regulator of chondrogenesis Slug transcription factor in osteoarthritic chondrocytes. METHODS: Lamin B1 and Slug proteins were analyzed in cartilage explants from normal subjects and OA patients by immunohistochemical technique. Their expression was confirmed on isolated chondrocytes both at passage 0 and passage 2 (de-differentiated chondrocytes) by immunofluorescence and western blot. Subsequently, we explored the "in vivo" binding of Slug on LMNB1 promoter by chromatin immunoprecipitation assay (ChIP). RESULTS: In this study we demonstrated that nuclear lamina protein Lamin B1 and anti-chondrogenic Slug transcription factor are upregulated in cartilage and OA chondrocytes. Furthermore, we found that Slug is "in vivo" recruited by LMNB1 gene promoter mostly when chondrocytes undergo de-differentiation or OA degeneration. CONCLUSIONS: We described for the first time a potential regulatory role of Slug on the LMNB1 gene expression in OA chondrocytes. These findings may have important implications for the study of premature senescence, and degeneration of cartilage, and may contribute to develop effective therapeutic strategies against signals supporting cartilage damage in different subsets of patients. Copyright 2015 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved. |
Maga G AIDS: la verità negata Book Il Pensiero Scientifico Editore, 2014, ISBN: 978-8849004809, (Vincitore del primo premio categoria saggi del Premio Letterario Nazionale Fanz Kafka - 2014). @book{CNRPRODOTTI303054,
title = {AIDS: la verità negata},
author = {Maga G},
url = {http://www.pensiero.it/ecomm/pc/viewPrd.asp?idproduct=651},
isbn = {978-8849004809},
year = {2014},
date = {2014-01-01},
publisher = {Il Pensiero Scientifico Editore},
series = {Informa},
abstract = {Ancora oggi esiste una corrente di opinione, tutt’altro che secondaria e sempre vitale, che afferma che l’AIDS non esiste e che l’HIV è un innocuo parassita. Ma negare l’AIDS significa sottovalutare la pericolosità dell’infezione da HIV e indurre i pazienti a rifiutare terapie in grado di salvare loro la vita. Quando l’opinione errata di alcuni compromette la salute e la sicurezza di altri, è necessario correggerla. Giovanni Maga dimostra, con un linguaggio comprensibile a tutti e attraverso la narrazione appassionata di tante storie di pazienti, medici e ricercatori, che respingere la relazione tra HIV e AIDS è un errore dalle drammatiche conseguenze. Documentando, inoltre, gli straordinari progressi fatti nel combattere questa malattia, il libro vuole essere un invito all’ottimismo e alla speranza. La difficoltà nell’accettare l’AIDS e, di conseguenza, la disponibilità a credere che non esista nascono spesso dalla disperazione di chi vive la sieropositività come una condanna senza appello. Ma la scienza arriverà inevitabilmente a sconfiggere l’HIV e questo libro ci spiega come e perché.},
note = {Vincitore del primo premio categoria saggi del Premio Letterario Nazionale Fanz Kafka - 2014},
keywords = {},
pubstate = {published},
tppubtype = {book}
}
Ancora oggi esiste una corrente di opinione, tutt’altro che secondaria e sempre vitale, che afferma che l’AIDS non esiste e che l’HIV è un innocuo parassita. Ma negare l’AIDS significa sottovalutare la pericolosità dell’infezione da HIV e indurre i pazienti a rifiutare terapie in grado di salvare loro la vita. Quando l’opinione errata di alcuni compromette la salute e la sicurezza di altri, è necessario correggerla. Giovanni Maga dimostra, con un linguaggio comprensibile a tutti e attraverso la narrazione appassionata di tante storie di pazienti, medici e ricercatori, che respingere la relazione tra HIV e AIDS è un errore dalle drammatiche conseguenze. Documentando, inoltre, gli straordinari progressi fatti nel combattere questa malattia, il libro vuole essere un invito all’ottimismo e alla speranza. La difficoltà nell’accettare l’AIDS e, di conseguenza, la disponibilità a credere che non esista nascono spesso dalla disperazione di chi vive la sieropositività come una condanna senza appello. Ma la scienza arriverà inevitabilmente a sconfiggere l’HIV e questo libro ci spiega come e perché. |
Focher F Due atei, un prete e un agnostico. Pranzo a casa Darwin Book Il Prato, Saonara, 2012, ISSN: 2037-9234. @book{CNRPRODOTTI177919,
title = {Due atei, un prete e un agnostico. Pranzo a casa Darwin},
author = {Focher F},
issn = {2037-9234},
year = {2012},
date = {2012-01-01},
volume = {41},
publisher = {Il Prato},
address = {Saonara},
series = {I cento talleri},
keywords = {},
pubstate = {published},
tppubtype = {book}
}
|
Di Pietro R; Centurione MA Elementi di Istologia Book Edises, Napoli, 2012, ISBN: 978 88 7959 743 2. @book{CNRPRODOTTI198989,
title = {Elementi di Istologia},
author = {{Di Pietro R} and Centurione MA},
isbn = {978 88 7959 743 2},
year = {2012},
date = {2012-01-01},
publisher = {Edises},
address = {Napoli},
keywords = {},
pubstate = {published},
tppubtype = {book}
}
|
Focher F Alexander von Humboldt. Schizzo biografico dal vivo Book Il Prato, Saonara, 2009, ISBN: 9788863360677. @book{CNRPRODOTTI135438,
title = {Alexander von Humboldt. Schizzo biografico dal vivo},
author = {Focher F},
isbn = {9788863360677},
year = {2009},
date = {2009-01-01},
volume = {26},
publisher = {Il Prato},
address = {Saonara},
keywords = {},
pubstate = {published},
tppubtype = {book}
}
|
Mondello C Multiple Pathways in cancer development. Book Transworld Research Network, Trivandrum, 2008, ISBN: 9788178953625. @book{CNRPRODOTTI135431,
title = {Multiple Pathways in cancer development.},
author = {Mondello C},
isbn = {9788178953625},
year = {2008},
date = {2008-01-01},
publisher = {Transworld Research Network},
address = {Trivandrum},
keywords = {},
pubstate = {published},
tppubtype = {book}
}
|
Focher F L'uomo che gettò nel panico Darwin. La vita e le scoperte di Alfred Russel Wallace. Book Bollati Boringhieri, Torino, 2006, ISBN: 9788833916705. @book{CNRPRODOTTI135417,
title = {L'uomo che gettò nel panico Darwin. La vita e le scoperte di Alfred Russel Wallace.},
author = {Focher F},
url = {https://www.bollatiboringhieri.it/libri/federico-focher-luomo-che-getto-nel-panico-darwin-9788833916705/},
isbn = {9788833916705},
year = {2006},
date = {2006-01-01},
publisher = {Bollati Boringhieri},
address = {Torino},
abstract = {Alfred Russel Wallace (1823-1913) fu uno dei massimi naturalisti vittoriani. I suoi interessi scientifici spaziavano dall’entomologia all’antropologia, dalla geologia alla glaciologia, dal mimetismo alla biogeografia evolutiva. Tuttavia, nonostante la sua vastissima produzione scientifica, egli viene generalmente ricordato solo per esser stato l’altro uomo che scopri la selezione naturale.
Pochi in realtà conoscono i suoi anni avventurosi trascorsi nelle foreste tropicali, l'eccellente lavoro di divulgazione del darwinismo, il sincero impegno sociale in difesa dei deboli e la sua fede nello spiritualismo, che lo portava a credere ottimisticamente in un continuo progresso morale e sociale dell’umanità.
La presente opera propone, inseriti in una sintetica cornice critica, i più famosi saggi evoluzionistici e antropologici di Wallace e alcuni significativi passi tratti dall'autobiografia e dagli scritti sociali.
Da queste pagine Wallace emerge non solo come un grande naturalista, ma anche, e sopratutto, come un grande uomo.},
keywords = {},
pubstate = {published},
tppubtype = {book}
}
Alfred Russel Wallace (1823-1913) fu uno dei massimi naturalisti vittoriani. I suoi interessi scientifici spaziavano dall’entomologia all’antropologia, dalla geologia alla glaciologia, dal mimetismo alla biogeografia evolutiva. Tuttavia, nonostante la sua vastissima produzione scientifica, egli viene generalmente ricordato solo per esser stato l’altro uomo che scopri la selezione naturale.
Pochi in realtà conoscono i suoi anni avventurosi trascorsi nelle foreste tropicali, l'eccellente lavoro di divulgazione del darwinismo, il sincero impegno sociale in difesa dei deboli e la sua fede nello spiritualismo, che lo portava a credere ottimisticamente in un continuo progresso morale e sociale dell’umanità.
La presente opera propone, inseriti in una sintetica cornice critica, i più famosi saggi evoluzionistici e antropologici di Wallace e alcuni significativi passi tratti dall'autobiografia e dagli scritti sociali.
Da queste pagine Wallace emerge non solo come un grande naturalista, ma anche, e sopratutto, come un grande uomo. |
Cavalli-Sforza LL; Moroni A; Zei G Consanguinity, Inbreeding and Genetic Drift in Italy Book 2004, ISBN: 9780691089928. @book{CNRPRODOTTI135395,
title = {Consanguinity, Inbreeding and Genetic Drift in Italy},
author = {Cavalli-Sforza LL and Moroni A and Zei G},
url = {https://press.princeton.edu/books/paperback/9780691089928/consanguinity-inbreeding-and-genetic-drift-in-italy-mpb-39},
isbn = {9780691089928},
year = {2004},
date = {2004-01-01},
abstract = {In 1951, the geneticist Luigi Luca Cavalli-Sforza was teaching in Parma when a student — a priest named Antonio Moroni — told him about rich church records of demography and marriages between relatives. After convincing the Church to open its records, Cavalli-Sforza, Moroni, and Gianna Zei embarked on a landmark study that would last fifty years and cover all of Italy. This book assembles and analyzes the team’s research for the first time.
Using blood testing as well as church records, the team investigated the frequency of consanguineous marriages and its use for estimating inbreeding and studying the relations between inbreeding and drift. They tested the importance of random genetic drift by studying population structure through demography of the last three centuries, using it to predict the spatial variation of frequencies of genetic markers. The authors find that drift-related genetic variation, including its stabilization by migration, is best predicted by computer simulation. They also analyze the usefulness and limits of the concept of deme for defining Mendelian populations. The genetic effect of consanguineous marriage on recessive genetic diseases and for the detection of dominance in metric characters are also studied.
Ultimately bringing together the many strands of their massive project, Cavalli-Sforza, Moroni, and Zei are able to map genetic drift in all of Italy’s approximately 8,000 communes and to demonstrate the relationship between each locality’s drift and various ecological and demographic factors. In terms of both methods and findings, their accomplishment is tremendously important for understanding human social structure and the genetic effects of drift and inbreeding.},
keywords = {},
pubstate = {published},
tppubtype = {book}
}
In 1951, the geneticist Luigi Luca Cavalli-Sforza was teaching in Parma when a student — a priest named Antonio Moroni — told him about rich church records of demography and marriages between relatives. After convincing the Church to open its records, Cavalli-Sforza, Moroni, and Gianna Zei embarked on a landmark study that would last fifty years and cover all of Italy. This book assembles and analyzes the team’s research for the first time.
Using blood testing as well as church records, the team investigated the frequency of consanguineous marriages and its use for estimating inbreeding and studying the relations between inbreeding and drift. They tested the importance of random genetic drift by studying population structure through demography of the last three centuries, using it to predict the spatial variation of frequencies of genetic markers. The authors find that drift-related genetic variation, including its stabilization by migration, is best predicted by computer simulation. They also analyze the usefulness and limits of the concept of deme for defining Mendelian populations. The genetic effect of consanguineous marriage on recessive genetic diseases and for the detection of dominance in metric characters are also studied.
Ultimately bringing together the many strands of their massive project, Cavalli-Sforza, Moroni, and Zei are able to map genetic drift in all of Italy’s approximately 8,000 communes and to demonstrate the relationship between each locality’s drift and various ecological and demographic factors. In terms of both methods and findings, their accomplishment is tremendously important for understanding human social structure and the genetic effects of drift and inbreeding. |
Gallorini M; Mencarelli N; Di Pietro N; di Giacomo V; Zara S; Ricci A; Rapino M; Piattelli A; Cipollina A; Cataldi A The Immunophenotype and the Odontogenic Commitment of Dental Pulp Stem Cells Co-Cultured with Macrophages Under Inflammatory Conditions Is Modulated by Complex Magnetic Fields Journal Article In: International journal of molecular sciences, vol. 26, iss. 1, pp. 48, 0000. @article{%a1.%Y__185,
title = {The Immunophenotype and the Odontogenic Commitment of Dental Pulp Stem Cells Co-Cultured with Macrophages Under Inflammatory Conditions Is Modulated by Complex Magnetic Fields},
author = {Gallorini M and Mencarelli N and Di Pietro N and di Giacomo V and Zara S and Ricci A and Rapino M and Piattelli A and Cipollina A and Cataldi A},
url = {https://www.mdpi.com/1422-0067/26/1/48},
doi = {10.3390/ijms26010048},
journal = {International journal of molecular sciences},
volume = {26},
issue = {1},
pages = {48},
abstract = {Dental inflammatory diseases remain a challenging clinical issue, whose causes and development are still not fully understood. During dental caries, bacteria penetrate the tooth pulp, causing pulpitis. To prevent pulp necrosis, it is crucial to promote tissue repair by recruiting immune cells, such as macrophages, able to secrete signal molecules for the pulp microenvironment and thus to recruit dental pulp stem cells (DPSCs) in the damaged site. To date, root canal therapy is the standard for dental caries, but alternative regenerative treatments are gaining attention. Complex Multifrequency Magnetoelectric Fields (CMFs) represent an interesting tool due to their potential anti-inflammatory activity. Against this background, the present work aims at investigating whether the CMF treatment might restore redox balance in a co-culture model of DPSCs and inflamed macrophages mimicking an inflammatory condition, like pulpitis. Results show that superoxide anion levels and markers related to the polarization of macrophages are modulated by the CMF treatment. In parallel, the use of CMFs discloses an impact on the odontogenic commitment of DPSCs, their immunophenotype being considerably modified. In conclusion, CMFs, by modulating the odontogenic commitment and the anti-inflammatory response of DPSCs, might represent a suitable therapeutic tool against pulpitis and, in general, towards dental inflammatory diseases.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Dental inflammatory diseases remain a challenging clinical issue, whose causes and development are still not fully understood. During dental caries, bacteria penetrate the tooth pulp, causing pulpitis. To prevent pulp necrosis, it is crucial to promote tissue repair by recruiting immune cells, such as macrophages, able to secrete signal molecules for the pulp microenvironment and thus to recruit dental pulp stem cells (DPSCs) in the damaged site. To date, root canal therapy is the standard for dental caries, but alternative regenerative treatments are gaining attention. Complex Multifrequency Magnetoelectric Fields (CMFs) represent an interesting tool due to their potential anti-inflammatory activity. Against this background, the present work aims at investigating whether the CMF treatment might restore redox balance in a co-culture model of DPSCs and inflamed macrophages mimicking an inflammatory condition, like pulpitis. Results show that superoxide anion levels and markers related to the polarization of macrophages are modulated by the CMF treatment. In parallel, the use of CMFs discloses an impact on the odontogenic commitment of DPSCs, their immunophenotype being considerably modified. In conclusion, CMFs, by modulating the odontogenic commitment and the anti-inflammatory response of DPSCs, might represent a suitable therapeutic tool against pulpitis and, in general, towards dental inflammatory diseases. |
