PubblicazioniPublications    
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
   HomePubblicazioni
Pubblicazioni IGM

Slug transcription factor and nuclear Lamin B1 are upregulated in osteoarthritic chondrocytes.

Autori

Piva R, Lambertini E, Manferdini C, Capanni C, Penolazzi L, Gabusi E, Paolella F, Lolli A, Angelozzi M, Lattanzi G, Lisignoli G.

Riferimenti

OSTEOARTHRITIS AND CARTILAGE 23(7) 1226-1230, 2015

Autori CNR

CAPANNI, LATTANZI

Moduli

Abstract

OBJECTIVE: To contribute to clarify molecular mechanisms supporting senescence and de-differentiation of chondrocytes in chondrocyte pathologies such as osteoarthritis (OA). Specifically, we investigated the relationship between the nuclear lamina protein Lamin B1 and the negative regulator of chondrogenesis Slug transcription factor in osteoarthritic chondrocytes. METHODS: Lamin B1 and Slug proteins were analyzed in cartilage explants from normal subjects and OA patients by immunohistochemical technique. Their expression was confirmed on isolated chondrocytes both at passage 0 and passage 2 (de-differentiated chondrocytes) by immunofluorescence and western blot. Subsequently, we explored the 'in vivo' binding of Slug on LMNB1 promoter by chromatin immunoprecipitation assay (ChIP). RESULTS: In this study we demonstrated that nuclear lamina protein Lamin B1 and anti-chondrogenic Slug transcription factor are upregulated in cartilage and OA chondrocytes. Furthermore, we found that Slug is 'in vivo' recruited by LMNB1 gene promoter mostly when chondrocytes undergo de-differentiation or OA degeneration. CONCLUSIONS: We described for the first time a potential regulatory role of Slug on the LMNB1 gene expression in OA chondrocytes. These findings may have important implications for the study of premature senescence, and degeneration of cartilage, and may contribute to develop effective therapeutic strategies against signals supporting cartilage damage in different subsets of patients. Copyright 2015 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

Link all articolo

http://www.sciencedirect.com/science/article/pii/S1063458415008560

Parole Chiave

Note

Indietro


Copyright © 2014 Home