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DNA-protein interaction dynamics at the Lamin B2 replication origin.

Autori

Puzzi L, Marchetti L, Peverali FA, Biamonti G, Giacca M.

Riferimenti

CELL CYCLE 14(1) 64-73, 2015

Autori CNR

BIAMONTI, PEVERALI

Moduli

Abstract

To date, a complete understanding of the molecular events leading to DNA replication origin activation in mammalian cells still remains elusive. In this work, we report the results of a high resolution chromatin immunoprecipitation study to detect proteins interacting with the human Lamin B2 replication origin. In addition to the pre-RC component ORC4 and to the transcription factors USF and HOXC13, we found that 2 components of the AP-1 transcription factor, c-Fos and c-Jun, are also associated with the origin DNA during the late G1 phase of the cell cycle and that these factors interact with ORC4. Both DNA replication and AP-1 factor binding to the origin region were perturbed by cell treatment with merbarone, a topoisomerase II inhibitor, suggesting that DNA topology is essential for determining origin function.

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