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Involvement of cyclic-nucleotide response element-binding family members in the radiation response of Ramos B lymphoma cells.

Autori

Di Nisio C, Sancilio S, Di Giacomo V, Rapino M, Sancillo L, Genovesi D, Di Siena A, Rana RA, Cataldi A, Di Pietro R.

Riferimenti

INTERNATIONAL JOURNAL OF ONCOLOGY 48(1) 28-36, 2016

Autori CNR

RAPINO

Moduli

Abstract

The aim of the present study was to investigate the role of Cyclic-nucleotide Response Element-Binding (CREB) family members and related nuclear transcription factors in the radiation response of human B lymphoma cell lines (Daudi and Ramos). Unlike the more radiosensitive Daudi cells, Ramos cells demonstrated only a moderate increase in early apoptosis after 3-5 Gy irradiation doses, which was detected with Annexin V/PI staining. Moreover, a significant and dose-dependent G2/M phase accumulation was observed in the same cell line at 24 h after both ionizing radiation (IR) doses. Western blot analysis showed an early increase in CREB protein expression that was still present at 3 h and more evident after 3 Gy IR in Ramos cells, along with the dose-dependent upregulation of p53 and NF-_B. These findings were consistent with real-time RT-PCR analysis that showed an early- and dose-dependent upregulation of NFKB1, IKBKB and XIAP gene expression. Unexpectedly, pre-treatment with SN50 did not increase cell death, but cell viability. Taken together, these findings let us hypothesise that the early induction and activation of NF-_B1 in Ramos cells could mediate necrotic cell death and be linked to other molecules belonging to CREB family and involved in the cell cycle regulation.

Link all articolo

http://www.spandidos-publications.com/ijo/48/1/28

Parole Chiave

Note

10.3892/ijo.2015.3238

Indietro


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