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Impact of ribonucleotide incorporation by DNA polymerases beta and lambda on oxidative base excision repair.

Autori

Crespan E, Furrer A, Rosinger M, Bertoletti F, Mentegari E, Chiapparini G, Imhof R, Ziegler N, Sturla SJ, Hubscher U, van Loon B, Maga G.

Riferimenti

NATURE COMMUNICATIONS 7 10805-, 2016

Autori CNR

CRESPAN, MAGA

Moduli

Abstract

Oxidative stress is a very frequent source of DNA damage. Many cellular DNA polymerases (Pols) can incorporate ribonucleotides (rNMPs) during DNA synthesis. However, whether oxidative stress-triggered DNA repair synthesis contributes to genomic rNMPs incorporation is so far not fully understood. Human specialized Pols _ and _ are the important enzymes involved in the oxidative stress tolerance, acting both in base excision repair and in translesion synthesis past the very frequent oxidative lesion 7,8-dihydro-8-oxoguanine (8-oxo-G). We found that Pol _, to a greater extent than Pol _ can incorporate rNMPs opposite normal bases or 8-oxo-G, and with a different fidelity. Further, the incorporation of rNMPs opposite 8-oxo-G delays repair by DNA glycosylases. Studies in Pol _- and _-deficient cell extracts suggest that Pol _ levels can greatly affect rNMP incorporation opposite oxidative DNA lesions.

Link all articolo

http://www.nature.com/ncomms/2016/160226/ncomms10805/full/ncomms10805.html

Parole Chiave

Note

10.1038/ncomms10805

Indietro


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