Miriana Cardano
Istituto di Genetica Molecolare “Luigi Luca Cavalli-Sforza”
Via Abbiategrasso, 207 – 27100 PAVIA
tel: +39 0382 546375
E-mail: miriana.cardano@igm.cnr.it
Curriculum Vitae – Download
Complete List of Publications – Download
Research Activity
Sexual dimorphism plays a critical role in determining cancer incidence and survival. The X-linked gene TSPY-Like 2 (TSPYL2) encodes for a protein involved in the regulation of cell cycle progression and DNA damage response (DDR), a complex of molecular pathways by which the cell is able to face DNA lesions that could lead to genomic instability and cancer initiation. The aim of our research is to further characterize the role of TSPYL2 in both male and female DDR and to clarify the mechanisms responsible for the occurrence and prognosis of sexually dimorphic tumors.
Technical Skills
Eukaryotic cell cultures, DNA extraction and electrophoresis analysis on agarose gel, RNA extraction, Cloning of plasmids and transfection (lipofectamine), Gene silencing, Production of knock out cell lines by CRISPR-Cas9, PCR, MTT assay, Colony formation assay, Real time q-PCR
Protein extraction and quantification, SDS-PAGE analysis, Western blotting analysis, Immunoprecipitation assay, Pull-down experiments, In vitro binding assay with recombinant proteins
Immunofluorescence experiments, Proximity Ligation Assay (PLA)
INFORMATICS SKILLS
MS Windows, OS Macintosh, Office (Word, PowerPoint, Excel), ImageJ, TotalLab, Bioinformatics tools (NCBI, EBI, UniProt, BLAST, Clustal Omega)
Research Project
Analysis of the gender specific role of TSPYL2 in the DNA damage response