Emmanuele Crespan

Istituto di Genetica Molecolare “Luigi Luca Cavalli-Sforza”
Via Abbiategrasso, 207 – 27100 PAVIA
tel: +39 0382 546354
fax: +39 0382 546370
E-mail: emmanuele.crespan@igm.cnr.it

Current Research Activity

RNase H2

With more than 107 ribonucleotides misincorporated in the human genome during replication, embedded ribonucleotides are the most abundant DNA lesions. The presence of the 2’-hydroxyl group makes misincorporated ribonucleotides prone to spontaneous hydrolysis, potentially resulting in the accumulation of strand breaks. Moreover, their presence may decrease the rate of DNA replication causing replicative fork stalling and, eventually, collapse. Ribonucleotide removal is initiated by Ribonuclease H2 (RNase H2), the key player in Ribonucleotide Excision Repair (RER). Its absence leads to embryonic lethality in mice, while mutations decreasing its activity cause the severe phenotype of the Aicardi–Goutières syndrome.

We are interested in the identification of factors that participate in RER using biochemical and cell biology approaches. We are also focusing our attention on the ability of RNase H2 to process ribonucleotides embedded in non-canonical DNA form and in the development of tools to map ribonucleotide embedded in the genome, with the aim to identify cold and hot spots for their misincorporation.

Alternative Lengthening of Telomeres

About 10% of cancers do not rely on telomerases activity as maintenance mechanism of telomeres (TMM), but on an alternative lengthening of telomeres (ALT) pathway. ALT-proficient cells show common features such as heterogenic and fluctuating telomere lengths, high levels of telomere recombination events, and abundant extrachromosomal telomeric repeat DNA. Despite a comprehensive view of the factors and the molecular mechanisms involved in ALT is still missing, mounting evidence led to a consensus on repair of double strand breaks (DSBs) originated from replication forks collapse as the leading cause of ALT. A homology directed process, involving the invasion and elongation of G-rich strand using the complementary strand as a template is also a consolidate characteristic of ALT mechanism. Different pathways has been proposed to explain TMM in ALT, all of which require enzymes able to catalyse DNA strand transfer and annealing reactions followed by DNA synthesis. So far, the identity of the DNA polymerases (Pols) involved in the elongation step of ALT has remained elusive.

By using multidisciplinary approaches (including biochemistry, cell biology, proteomics, imaging, CRISPR libraries, and animal models) we investigate of the roles of specialised DNA polymerases in ALT mechanism and, in general, in the reconstruction of the molecular steps occurring in ALT initiation. The goal of this research is the identification of enzyme(s) essential in ALT process that to be considered as targets for new therapeutic approaches.

CANVAS

Co-occurrence of cerebellar Ataxia with Neuropathy and Vestibular Areflexia Syndrome (CANVAS) is a rare, adult-onset, slowly progressive neurological disorder characterized by imbalance, sensory neuropathy (neuronopathy), bilateral vestibulopathy, chronic cough, and occasionally autonomic dysfunction. The genetic cause of the disease was very recently discovered and consists of biallelic AAGGG repeat expansions in the second intron of replication factor C subunit 1 (RFC1). The mechanism leading to symptoms onset is not yet understood.

To understand the mechanism driving CANVAS onset, we aim to characterize RFC1 activity on CANVAS derived patient cell lines through biochemistry and cellular based assays.

Repair DNA polymerases.

DNA repair is crucial to maintain genomic stability, with eepair DNA polymerases, as well as translesion polymerases playing a pivotal role. We have focus our attention on the study of the enzymatic properties and cellular roles of DNA polymerases in response to DNA damage in an attempt to identify specific activities and molecular processes that could be targets for innovative therapies. In particular, we identified and characterized selective inhibitors of specific polymerases, including compounds that can be incorporated specifically against certain types of DNA damages, such as abasic sites, to be used as markers for these lesions. The same research has led to the elucidation of the role of repair polymerases and other accessory factors for the faithful bypass of various lesions. In particular, we found that DNA polymerase Lambda is specifically recruited for the faithful bypass of the mutagenic, and most common oxidised lesion, 8-oxoG.We also explored the contribution of repair polymerases in the expansion of tri-nucleotides repeats that are characteristic of different neurodegenerative diseases, suggesting a possible role of polymerase beta in Poly-Q disorders.

Drugs development.

Part of our research is focused on the characterization of new inhibitors of molecular targets involved in viral, neurodegenerative, and neoplastic diseases. In particular, we are interested in the evaluation of the inhibition potency, toxicity, and mechanism of action of new drug candidates through in vitro assays using recombinant proteins as well as cellular models. We have been involved in the characterization of molecules targeting essential factors in the replication of different viruses including HIV-1, HCV, Zika Virus, enteroviruses, and Dengue virus. We are also collaborating with several groups on the evaluation of small molecules as inhibitors of kinases responsible of the onset and progression of specific cancers or targets of viral or bacterial infections (host targets), or involved in neurodegenerative diseases progression.

Orcid id:                       0000-0003-0597-6929

 


Recent Publications

2023

Poggialini F; Vagaggini C; Brai A; Pasqualini C; Crespan E; Maga G; Perini C; Cabella N; Botta L; Musumeci F; Frosini M; Schenone S; Dreassi E

Biological Evaluation and In Vitro Characterization of ADME Profile of In-House Pyrazolo[3,4- d]pyrimidines as Dual Tyrosine Kinase Inhibitors Active against Glioblastoma Multiforme Journal Article

In: Pharmaceutics, vol. 15, iss. 2, pp. 453, 2023.

Abstract | Links | BibTeX

Contadini C; Cirotti C; Carbone A; Norouzi M; Cianciusi A; Crespan E; Perini C; Maga G; Barilà D; Musumeci F; Schenone S

Identification and Biological Characterization of the Pyrazolo[3,4- d]pyrimidine Derivative SI388 Active as Src Inhibitor Journal Article

In: Pharmaceuticals - Basel, vol. 16, iss. 7, pp. 958, 2023.

Abstract | Links | BibTeX

Dominik N; Magri S; Currò R; Abati E; Facchini S; Corbetta M; MacPherson H; Di Bella D; Sarto E; Stevanovski I; Chintalaphani SR; Akcimen F; Manini A; Vegezzi E; Quartesan I; Montgomery KA; Pirota V; Crespan E; Perini C; Grupelli GP; Tomaselli PJ; Marques W; Genomics England Research Consortium; Shaw J; Polke J; Salsano E; Fenu S; Pareyson D; Pisciotta C; Tofaris GK; Nemeth AH; Ealing J; Radunovic A; Kearney S; Kumar KR; Vucic S; Kennerson M; Reilly MM; Houlden H; Deveson I; Tucci A; Taroni F; Cortese A

Normal and pathogenic variation of RFC1 repeat expansions: implications for clinical diagnosis Journal Article

In: Brain, vol. 146, iss. 12, pp. 5060-5069, 2023.

Abstract | Links | BibTeX

Ronco R; Perini C; Currò R; Dominik N; Facchini S; Gennari A; Simone R; Stuart S; Nagy S; Vegezzi E; Quartesan I; El-Saddig A; Lavin T; Tucci A; Szymura A; Novis De Farias LE; Gary A; Delfeld M; Kandikatla P; Niu N; Tawde S; Shaw J; Polke J; Reilly MM; Wood NW; Crespan E; Gomez C; Chen JYH; Schmahmann JD; Gosal D; Houlden H; Das S; Cortese A

Truncating Variants in RFC1 in Cerebellar Ataxia, Neuropathy, and Vestibular Areflexia Syndrome Journal Article

In: NEUROLOGY, vol. 100, iss. 5, pp. e543-e554, 2023.

Abstract | Links | BibTeX

2022

Di Maria S; Picarazzi F; Mori M; Cianciusi A; Carbone A; Crespan E; Perini C; Sabetta S; Deplano S; Poggialini F; Molinari A; Aronne R; Maccioni E; Maga G; Angelucci A; Schenone S; Musumeci F; Dreassi E

Novel pyrazolo[3,4-d]pyrimidines as dual Src/Bcr-Abl kinase inhibitors: Synthesis and biological evaluation for chronic myeloid leukemia treatment. Journal Article

In: Bioorganic chemistry, vol. 128, pp. 106071, 2022.

Abstract | Links | BibTeX

Princiotto S; Musso L; Manetti F; Marcellini V; Maga G; Crespan E; Perini C; Zaffaroni N; Beretta GL; Dallavalle S

Synthesis and biological activity evaluation of 3-(hetero) arylideneindolin-2-ones as potential c-Src inhibitors Journal Article

In: Journal of enzyme inhibition and medicinal chemistry, vol. 37, iss. 1, pp. 2382-2394, 2022.

Abstract | Links | BibTeX

2021

Mentegari E; Bertoletti F; Kissova M; Zucca E; Galli S; Tagliavini G; Garbelli A; Maffia A; Bione S; Ferrari E; d'Adda di Fagagna F; Francia S; Sabbioneda S; Chen LY; Lingner J; Bergoglio V; Hoffmann JS; Hubscher U; Crespan E; Maga G

A Role for Human DNA Polymerase lambda in Alternative Lengthening of Telomeres Journal Article

In: International journal of molecular sciences, vol. 22, no. 5, pp. 2365, 2021.

Abstract | Links | BibTeX

Grazia Martina M; Vicenti I; Bauer L; Crespan E; Rango E; Boccuto A; Olivieri N; Incerti M; Zwaagstra M; Allodi M; Bertoni S; Dreassi E; Zazzi M; van Kuppeveld FJM; Maga G; Radi M

Bithiazole Inhibitors of Phosphatidylinositol 4-Kinase (PI4KIIIBETA) as Broad-Spectrum Antivirals Blocking the Replication of SARS-CoV-2, Zika Virus, and Human Rhinoviruses Journal Article

In: ChemMedChem, vol. 16, iss. 23, no. 3548, pp. 3552, 2021.

Abstract | Links | BibTeX

Dede M; Napolitano S; Melati A; Pirota V; Maga G; Crespan E

High Flexibility of RNaseH2 Catalytic Activity with Respect to Non-Canonical DNA Structures Journal Article

In: International journal of molecular sciences, vol. 22, no. 10, pp. 5201, 2021.

Abstract | Links | BibTeX

Pirota V; Lunghi E; Benassi A; Crespan E; Freccero M; Doria F

Selective Binding and Redox-Activity on Parallel G-Quadruplexes by Pegylated Naphthalene Diimide-Copper Complexes Journal Article

In: Molecules, vol. 26, no. 16, pp. 5025, 2021.

Abstract | Links | BibTeX

Vicenti I; Martina MG; Boccuto A; De Angelis M; Giavarini G; Dragoni F; Marchi S; Trombetta CM; Crespan E; Maga G; Eydoux C; Decroly E; Montomoli E; Nencioni L; Zazzi M; Radi M

System-oriented optimization of multi-target 2,6-diaminopurine derivatives: Easily accessible broad-spectrum antivirals active against flaviviruses, influenza virus and SARS-CoV-2. Journal Article

In: European journal of medicinal chemistry, vol. 224, pp. 113683, 2021.

Abstract | Links | BibTeX

2020

Nalli M; Armijos Rivera JI; Masci D; Coluccia A; Badia R; Riveira-Munoz E; Brambilla A; Cinquina E; Turriziani O; Falasca F; Catalano M; Limatola C; Este JA; Maga G; Silvestri R; Crespan E; La Regina G

New indolylarylsulfone non-nucleoside reverse transcriptase inhibitors show low nanomolar inhibition of single and double HIV-1 mutant strains Journal Article

In: European journal of medicinal chemistry, vol. 208, pp. 112696, 2020.

Abstract | Links | BibTeX

2019

Nawrozkij M; Forgione M; Yablokov AS; Lucidi A; Tomaselli D; Patsilinakos A; Panella C; Hailu GS; Kirillov IA; Badia R; Riveira Muñoz E; Crespan E; Armijos-Rivera JI; Cirilli R; Ragno R; Este JA; Maga G; Mai A; Rotili D

Effect of alpha-Methoxy Substitution on the anti-HIV Activity of Dihydropyrimidin-4(3H)-ones. Journal Article

In: Journal of medicinal chemistry, vol. 62, no. 2, pp. 604-621, 2019.

Abstract | Links | BibTeX

Fallacara AL; Passannanti R; Mori M; Iovenitti G; Musumeci F; Greco C; Crespan E; Kissova M; Maga G; Tarantelli C; Spriano F; Gaudio E; Bertoni F; Botta M; Schenone S

Identification of a new family of pyrazolo[3,4-d]pyrimidine derivatives as multitarget Fyn-Blk-Lyn inhibitors active on B- and T-lymphoma cell lines. Journal Article

In: European journal of medicinal chemistry, vol. 181, pp. 111545, 2019.

Abstract | Links | BibTeX

Geronikaki A; Petrou A; Kartsev V; Eleftheriou P; Boga R; Bartolo B; Crespan E; Franco G; Maga G

Molecular docking, design, synthesis and biological evaluation of novel 2,3-aryl-thiazolidin-4-ones as potent NNRTIs. Journal Article

In: SAR and QSAR in environmental research, vol. 30, no. 10, pp. 697-714, 2019.

Abstract | Links | BibTeX

Tassini S; Langron E; Delang L; Mirabelli C; Lanko K; Crespan E; Kissova M; Tagliavini G; Fontò G; Bertoni S; Palese S; Giorgio C; Ravanetti F; Ragionieri L; Zamperini C; Mancini A; Dreassi E; Maga G; Vergani P; Neyts J; Radi M

Multitarget CFTR Modulators Endowed with Multiple Beneficial Side Effects for Cystic Fibrosis Patients: Toward a Simplified Therapeutic Approach Journal Article

In: Journal of medicinal chemistry, vol. 62, no. 23, pp. 10833-10847, 2019.

Abstract | Links | BibTeX

2018

Molinari A; Fallacara AL; Di Maria S; Zamperini C; Poggialini F; Musumeci F; Schenone S; Angelucci A; Colapietro A; Crespan E; Kissova M; Maga G; Botta M

Efficient optimization of pyrazolo[3,4-d]pyrimidines derivatives as c-Src kinase inhibitors in neuroblastoma treatment. Journal Article

In: Bioorganic & medicinal chemistry letters, vol. 28, no. 21, pp. 3454-3457, 2018.

Abstract | Links | BibTeX

Kaptein SJF; Vincetti P; Crespan E; Rivera JIA; Costantino G; Maga G; Neyts J; Radi M

Identification of Broad-Spectrum Dengue/Zika Virus Replication Inhibitors by Functionalization of Quinoline and 2,6-Diaminopurine Scaffolds. Journal Article

In: ChemMedChem, vol. 13, no. 14, pp. 1371-1376, 2018.

Abstract | Links | BibTeX

2017

Famiglini V; La Regina G; Coluccia A; Masci D; Brancale A; Badia R; Riveira-Munoz E; Esté JA; Crespan E; Brambilla A; Maga G; Catalano M; Limatola C; Formica FR; Cirilli R; Novellino E; Silvestri R

Chiral Indolylarylsulfone Non-Nucleoside Reverse Transcriptase Inhibitors as New Potent and Broad Spectrum Anti-HIV-1 Agents. Journal Article

In: Journal of medicinal chemistry, vol. 60, no. 15, pp. 6528-6547, 2017.

Abstract | Links | BibTeX

Tassini S; Sun L; Lanko K; Crespan E; Langron E; Falchi F; Kissova M; Armijos-Rivera JI; Delang L; Mirabelli C; Neyts J; Pieroni M; Cavalli A; Costantino G; Maga G; Vergani P; Leyssen P; Radi M

Discovery of Multitarget Agents Active as Broad-Spectrum Antivirals and Correctors of Cystic Fibrosis Transmembrane Conductance Regulator for Associated Pulmonary Diseases. Journal Article

In: Journal of medicinal chemistry, vol. 60, no. 4, pp. 1400-1416, 2017.

Abstract | Links | BibTeX

Garbelli A; Riva V; Crespan E; Maga G

How to win the HIV-1 drug resistance hurdle race: running faster or jumping higher? Journal Article

In: Biochemical journal, vol. 474, no. 10, pp. 1559-1577, 2017.

Abstract | Links | BibTeX

Musumeci F; Fallacara AL; Brullo C; Grossi G; Botta L; Calandro P; Chiariello M; Kissova M; Crespan E; Maga G; Schenone S

Identification of new pyrrolo[2,3-d]pyrimidines as Src tyrosine kinase inhibitors in vitro active against Glioblastoma. Journal Article

In: European journal of medicinal chemistry, vol. 127, pp. 369-378, 2017.

Abstract | Links | BibTeX

Mentegari E; Crespan E; Bavagnoli L; Kissova M; Bertoletti F; Sabbioneda S; Imhof R; Sturla SJ; Nilforoushan A; Hubscher U; van Loon B; Maga G

Ribonucleotide incorporation by human DNA polymerase eta impacts translesion synthesis and RNase H2 activity. Journal Article

In: Nucleic Acids Research, vol. 45, no. 5, pp. 2600-2614, 2017.

Abstract | Links | BibTeX

Meroni A; Mentegari E; Crespan E; Muzi-Falconi M; Lazzaro F; Podestà A

The Incorporation of Ribonucleotides Induces Structural and Conformational Changes in DNA. Journal Article

In: Biophysical journal, vol. 113, no. 7, pp. 1373-1382, 2017.

Abstract | Links | BibTeX

2016

Radi M; Schneider R; Fallacara AL; Botta L; Crespan E; Tintori C; Maga G; Kissova M; Calgani A; Richters A; Musumeci F; Rauh D; Schenone S

A cascade screening approach for the identification of Bcr-Abl myristate pocket binders active against wild type and T315I mutant. Journal Article

In: Bioorganic & Medicinal Chemistry Letters, vol. 26, no. 15, pp. 3436-3440, 2016.

Abstract | Links | BibTeX

Tintori C; Brai A; Dasso Lang MC; Deodato D; Greco AM; Bizzarri BM; Cascone L; Casian A; Zamperini C; Dreassi E; Crespan E; Maga G; Vanham G; Ceresola E; Canducci F; Arien KK; Botta M

Development and in Vitro Evaluation of a Microbicide Gel Formulation for a Novel Non-Nucleoside Reverse Transcriptase Inhibitor Belonging to the N-Dihydroalkyloxybenzyloxopyrimidines (N-DABOs) Family. Journal Article

In: Journal of medicinal chemistry, vol. 59, no. 6, pp. 2747-2759, 2016.

Abstract | Links | BibTeX

Goodson WH 3rd; Lowe L; Carpenter DO; Gilbertson M; Manaf Ali A; Lopez de Cerain Salsamendi A; Lasfar A; Carnero A; Azqueta A; Amedei A; Charles AK; Collins AR; Ward A; Salzberg AC; Colacci A; Olsen AK; Berg A; Barclay BJ; Zhou BP; Blanco-Aparicio C; Baglole CJ; Dong C; Mondello C; Hsu CW; Naus CC; Yedjou C; Curran CS; Laird DW; Koch DC; Carlin DJ; Felsher DW; Roy D; Brown DG; Ratovitski E; Ryan EP; Corsini E; Rojas E; Moon EY; Laconi E; Marongiu F; Al-Mulla F; Chiaradonna F; Darroudi F; Martin FL; Van Schooten FJ; Goldberg GS; Wagemaker G; Nangami G; Calaf GM; Williams G; Wolf GT; Koppen G; Brunborg G; Kim Lyerly H; Krishnan H; Ab Hamid H; Yasaei H; Sone H; Kondoh H; Salem HK; Hsu HY; Park HH; Koturbash I; Miousse IR; Scovassi AI; Klaunig JE; Vondráček J; Raju J; Roman J; Wise JP Sr; Whitfield JR; Woodrick J; Christopher JA; Ochieng J; Martinez-Leal JF; Weisz J; Kravchenko J; Sun J; Prudhomme KR; Narayanan KB; Cohen-Solal KA; Moorwood K; Gonzalez L; Soucek L; Jian L; D'Abronzo LS; Lin LT; Li L; Gulliver L; McCawley LJ; Memeo L; Vermeulen L; Leyns L; Zhang L; Valverde M; Khatami M; Romano MF; Chapellier M; Williams MA; Wade M; Manjili MH; Lleonart M; Xia M; Gonzalez MJ; Karamouzis MV; Kirsch-Volders M; Vaccari M; Kuemmerle NB; Singh N; Cruickshanks N; Kleinstreuer N; van Larebeke N; Ahmed N; Ogunkua O; Krishnakumar PK; Vadgama P; Marignani PA; Ghosh PM; Ostrosky-Wegman P; Thompson P; Dent P; Heneberg P; Darbre P; Sing Leung P; Nangia-Makker P; Cheng QS; Robey RB; Al-Temaimi R; Roy R; Andrade-Vieira R; Sinha RK; Mehta R; Vento R; Di Fiore R; Ponce-Cusi R; Dornetshuber-Fleiss R; Nahta R; Castellino RC; Palorini R; Abd Hamid R; Langie SA; Eltom S; Brooks SA; Ryeom S; Wise SS; Bay SN; Harris SA; Papagerakis S; Romano S; Pavanello S; Eriksson S; Forte S; Casey SC; Luanpitpong S; Lee TJ; Otsuki T; Chen T; Massfelder T; Sanderson T; Guarnieri T; Hultman T; Dormoy V; Odero-Marah V; Sabbisetti V; Maguer-Satta V; Rathmell WK; Engström W; Decker WK; Bisson WH; Rojanasakul Y; Luqmani Y; Chen Z; Hu Z Llona-Minguez S; Hoglund A; Jacques SA; Johansson L; Calderon-Montano JM; Claesson M; Loseva O; Valerie NC; Lundbäck T; Piedrafita J; Maga G; Crespan E; Meijer L; Burgos Morón E; Baranczewski P; Hagbjork AL; Svensson R; Wiita E; Almlof I; Visnes T; Jeppsson F; Sigmundsson K; Jensen AJ; Artursson P; Jemth AS; Stenmark P; Warpman Berglund U; Scobie M; Helleday T

Discovery of the First Potent and Selective Inhibitors of Human dCTP Pyrophosphatase 1. Journal Article

In: Journal of medicinal chemistry, vol. 59, no. 3, pp. 1140-1148, 2016.

Abstract | Links | BibTeX

Mentegari E; Kissova M; Bavagnoli L; Maga G; Crespan E

DNA Polymerases lambda and beta: The Double-Edged Swords of DNA Repair. Journal Article

In: Genes (Basel), vol. 7, no. 9, pp. e57, 2016.

Abstract | Links | BibTeX

Crespan E; Furrer A; Rösinger M; Bertoletti F; Mentegari E; Chiapparini G; Imhof R; Ziegler N; Sturla SJ; Hubscher U; van Loon B; Maga G

Impact of ribonucleotide incorporation by DNA polymerases beta and lambda on oxidative base excision repair. Journal Article

In: Nature Communications, vol. 7, pp. 10805, 2016.

Abstract | Links | BibTeX

El-Moghazy SM; George RF; Osman EE; Elbatrawy AA; Kissova M; Colombo A; Crespan E; Maga G

Novel pyrazolo[3,4-d]pyrimidines as dual Src-Abl inhibitors active against mutant form of Abl and the leukemia K-562 cell line. Journal Article

In: European Journal of Medicinal Chemistry, vol. 123, pp. 1-13, 2016.

Abstract | Links | BibTeX

Kissova M; Maga G; Crespan E

The human tyrosine kinase Kit and its gatekeeper mutant T670I, show different kinetic properties: Implications for drug design. Journal Article

In: Bioorganic & Medicinal Chemistry, vol. 24, no. 19, pp. 4555-4532, 2016.

Abstract | Links | BibTeX

2015

Tintori C; Fallacara AL; Radi M; Zamperini C; Dreassi E; Crespan E; Maga G; Schenone S; Musumeci F; Brullo C; Richters A; Gasparrini F; Angelucci A; Festuccia C; Delle Monache S; Rauh D; Botta M

Combining X-ray Crystallography and Molecular Modeling toward the Optimization of Pyrazolo[3,4-d]pyrimidines as Potent c-Src Inhibitors Active in Vivo against Neuroblastoma. Journal Article

In: Journal of Medicinal Chemistry, vol. 58, no. 1, pp. 347-361, 2015.

Abstract | Links | BibTeX

Vincetti P; Caporuscio F; Kaptein S; Gioiello A; Mancino V; Suzuki Y; Yamamoto N; Crespan E; Lossani A; Maga G; Rastelli G; Castagnolo D; Neyts J; Leyssen P; Costantino G; Radi M

Discovery of Multitarget Antivirals Acting on Both the Dengue Virus NS5-NS3 Interaction and the Host Src/Fyn Kinases. Journal Article

In: Journal of Medicinal Chemistry, vol. 58, no. 12, 2015.

Abstract | Links | BibTeX

Crespan E; Hübscher U; Maga G

Expansion of CAG triplet repeats by human DNA polymerases λ and β in vitro, is regulated by flap endonuclease 1 and DNA ligase 1. Journal Article

In: DNA Repair, vol. 29, pp. 101-111, 2015.

Abstract | Links | BibTeX

Tintori C; La Sala G; Vignaroli G; Botta L; Fallacara AL; Falchi F; Radi M; Zamperini C; Dreassi E; Dello Iacono L; Orioli D; Biamonti G; Garbelli M; Lossani A; Gasparrini F; Tuccinardi T; Laurenzana I; Angelucci A; Maga G; Schenone S; Brullo C; Musumeci F; Desogus A; Crespan E; Botta M

Studies on the ATP Binding Site of Fyn Kinase for the Identification of New Inhibitors and Their Evaluation as Potential Agents against Tauopathies and Tumors. Journal Article

In: Journal of Medicinal Chemistry, vol. 58, no. 11, pp. 4590-4609, 2015.

Abstract | Links | BibTeX

Spallarossa A; Caneva C; Caviglia M; Alfei S; Butini S; Campiani G; Gemma S; Brindisi M; Zisterer DM; Bright SA; Williams CD; Crespan E; Maga G; Sanna G; Delogu I; Collu G; Loddo R

Unconventional Knoevenagel-type indoles: Synthesis and cell-based studies for the identification of pro-apoptotic agents. Journal Article

In: European Journal of Medicinal Chemistry, vol. 102, pp. 648-660, 2015.

Abstract | Links | BibTeX