Anna Di Matteo

Istituto di Genetica Molecolare “Luigi Luca Cavalli-Sforza”
Via Abbiategrasso, 207 – 27100 PAVIA
tel: +39 0382 546375
E-mail: anna.dimatteo@igm.cnr.it


Curriculum Vitae – Download

Publications – Download


Research Activity

We study the biological relevance of alternative splicing (AS) for the control of physiological and tumoral angiogenesis. The main goal of this research is to identify and characterize signaling pathways that play pivotal role(s) for vascular development and function in health and disease and to identify new AS targets selectively expressed on the surface of endothelial cells of the tumor vasculature.


Research expertise 

Molecular biology: Cloning procedures, plasmid production and purification. RNA extraction and purification from cells, tissues and Zebrafish embryos. PCR, qPCR, PCR mediated mutagenesis, RT-PCR. Electrophoresis on agarose gel, LM gel and acrylamide gel. DNA, RNA and protein quantification. UV-RIP, iCLIP and PAR-CLIP techniques. SDS-PAGE and Western blotting. Immunofluorescence on mammalian cells.

Cellular Biology: Eukaryotic cell culture. Eukaryotic cell transfection.

Mouse Biology: Enucleation of murine eyes and dissection of the retina. in situ hybridization on whole-mount mouse retina.

Microscopy: Optical and Fluorescent Microscopy.

Bioinformatic tools and Software: NCBI, Ensembl, UCSC, BLAST, DAVID, Primer3, ExonMine, Uniprot, InterPro, Expasy, IGV (Integrative Genomic Viewer). MS Office. Photoshop. NIH ImageJ. Illustrator. GraphPad Prism.

Research activity: Experience in writing scientific papers and project proposals.

 

Research Project

Post-transcriptional regulation of angiogenesis


Publications

Articles

  • Belloni E*, Di Matteo A*, Pradella D*, Vacca M, Wyatt CDR, Alfieri R, Maffia A, Sabbioneda S, Ghigna C. Gene Expression Profiles Controlled by the Alternative Splicing Factor Nova2 in Endothelial Cells. Cells. 2019. 8(12). pii: E1498. doi: 10.3390/cells8121498.
    * These authors contributed equally to this work.
  • Angiolini F, Belloni E, Giordano M, Campioni M, Forneris F, Paronetto MP, Lupia M, Brandas C, Pradella D, Di Matteo A, Giampietro C, Jodice G, Luise C, Bertalot G, Freddi S, Malinverno M, Irimia M, Moulton JD, Summerton J, Chiapparino A, Ghilardi C, Giavazzi R, Nyqvist D, Gabellini D, Dejana E, Cavallaro U, Ghigna C. A novel L1CAM isoform with angiogenic activity generated by NOVA2-mediated alternative splicing. Elife. 2019. 8. pii: e44305. doi:10.7554/eLife.44305.
  • Giampietro C, Deflorian G, Gallo S, Di Matteo A, Pradella D, Bonomi S, Belloni E, Nyqvist D, Quaranta V, Confalonieri S, Bertalot G, Orsenigo F, Pisati F, Ferrero E, Biamonti G, Fredrickx E, Taveggia C, Wyatt CD, Irimia M, Di Fiore PP, Blencowe BJ, Dejana E, Ghigna C. The alternative splicing factor Nova2 regulates vascular development and lumen formation. Nat Commun. 2015. 6:8479. doi:10.1038/ncomms9479.
  • Bonomi S, Di Matteo A, Buratti E, Cabianca DS, Baralle FE, Ghigna C, Biamonti G. (2013). HnRNP A1 controls a splicing regulatory circuit promoting mesenchymal-to-epithelial transition. Nucleic Acids Res. 41: 8665-79.

Reviews

  • Biamonti G, Amato A*, Belloni E*, Di Matteo A*, Infantino L, Pradella D, Ghigna C. Alternative splicing in Alzheimer’s disease. Aging Clin Exp Res. 2019. Published online ahead of print. doi:10.1007/s40520-019-01360-x.
    * These authors contributed equally to this work.
  • Frisone P, Pradella D, Di Matteo A, Belloni E, Ghigna C, Paronetto MP. SAM68: Signal Transduction and RNA Metabolism in Human Cancer. Biomed Res Int. 2015. 2015:528954. doi:10.1155/2015/528954

Chapters in book

  • Paronetto MP, Gallo S, Di Matteo A, Ghigna C. Alternative Pre-mRNA Processing in Cancer Progression: Clinical Significance and Therapeutic Implications. 2014. Global Journal of Human Genetics & Gene Therapy. 2:1-16.